Review



anti cleaved caspase 9  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc anti cleaved caspase 9
    Anti Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 8887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Caspase-9+Antibody/pmc12666026-25-5-16
    Average 97 stars, based on 8887 article reviews
    anti cleaved caspase 9 - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesis.
    Article Snippet: Next, samples were mixed with Laemmli buffer, boiled at 95°C for 10 min, further loaded onto SDS–polyacrylamide gel electrophoresis gels, and transferred to a polyvinylidene difluoride membrane (Millipore). .. After blocking, proteins were probed by the following primary antibodies: caspase- 1 (1:2000; VIB Core), caspase- 3 (1:1000; 9662, CST), caspase- 7 (1:1000; 9492S, CST), caspase- 8 (1:1000; MAB3429, Abnova), clcaspase- 8 (1:1000; 9429, CST), caspase- 9 (1:1000; 9508, CST), caspase- 11 (1:200; NB120- 10454, NOVUS), pRIPK1 (Ser166) (1:1000; 53286S, CST), RIPK1 (1:1000; 3493, CST), pRIPK3 (Thr231/Ser232) (1:1000; 57220, CST), pMLKL (Ser345) (1:1000; 37333, CST), MLKL (1:1000; MABC604, Millipore), GSDMD (1:1000; ab209845, Abcam), GSDME (1:500; 13075- 1- AP, Proteintech), GPX4 (1:5000; ab125066, Abcam), vinculin (1:200; V9131, Sigma- Aldrich), β- tubulin HRP (1:10,000; ab21058, Abcam), and β- actin HRP (1:5000; 5125, CST); followed by HRP- conjugated secondary antibody (GE Healthcare) incubation. .. Detection was performed with the Western Lightning chemiluminescent reagent plus kit (NEL105001EA, PerkinElmer).

    Article Title: Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesis
    Article Snippet: Next, samples were mixed with Laemmli buffer, boiled at 95°C for 10 min, further loaded onto SDS–polyacrylamide gel electrophoresis gels, and transferred to a polyvinylidene difluoride membrane (Millipore). .. After blocking, proteins were probed by the following primary antibodies: caspase-1 (1:2000; VIB Core), caspase-3 (1:1000; 9662, CST), caspase-7 (1:1000; 9492S, CST), caspase-8 (1:1000; MAB3429, Abnova), clcaspase-8 (1:1000; 9429, CST), caspase-9 (1:1000; 9508, CST), caspase-11 (1:200; NB120-10454, NOVUS), pRIPK1 (Ser 166 ) (1:1000; 53286S, CST), RIPK1 (1:1000; 3493, CST), pRIPK3 (Thr 231 /Ser 232 ) (1:1000; 57220, CST), pMLKL (Ser 345 ) (1:1000; 37333, CST), MLKL (1:1000; MABC604, Millipore), GSDMD (1:1000; ab209845, Abcam), GSDME (1:500; 13075-1-AP, Proteintech), GPX4 (1:5000; ab125066, Abcam), vinculin (1:200; V9131, Sigma-Aldrich), β-tubulin HRP (1:10,000; ab21058, Abcam), and β-actin HRP (1:5000; 5125, CST); followed by HRP-conjugated secondary antibody (GE Healthcare) incubation. .. Detection was performed with the Western Lightning chemiluminescent reagent plus kit (NEL105001EA, PerkinElmer).

    Incubation:

    Article Title: Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesis.
    Article Snippet: Next, samples were mixed with Laemmli buffer, boiled at 95°C for 10 min, further loaded onto SDS–polyacrylamide gel electrophoresis gels, and transferred to a polyvinylidene difluoride membrane (Millipore). .. After blocking, proteins were probed by the following primary antibodies: caspase- 1 (1:2000; VIB Core), caspase- 3 (1:1000; 9662, CST), caspase- 7 (1:1000; 9492S, CST), caspase- 8 (1:1000; MAB3429, Abnova), clcaspase- 8 (1:1000; 9429, CST), caspase- 9 (1:1000; 9508, CST), caspase- 11 (1:200; NB120- 10454, NOVUS), pRIPK1 (Ser166) (1:1000; 53286S, CST), RIPK1 (1:1000; 3493, CST), pRIPK3 (Thr231/Ser232) (1:1000; 57220, CST), pMLKL (Ser345) (1:1000; 37333, CST), MLKL (1:1000; MABC604, Millipore), GSDMD (1:1000; ab209845, Abcam), GSDME (1:500; 13075- 1- AP, Proteintech), GPX4 (1:5000; ab125066, Abcam), vinculin (1:200; V9131, Sigma- Aldrich), β- tubulin HRP (1:10,000; ab21058, Abcam), and β- actin HRP (1:5000; 5125, CST); followed by HRP- conjugated secondary antibody (GE Healthcare) incubation. .. Detection was performed with the Western Lightning chemiluminescent reagent plus kit (NEL105001EA, PerkinElmer).

    Article Title: Antioxidant and Anti-Aging Effects of Porphyra-334 Produced from Saccharomyces cerevisiae in Human Skin Models.
    Article Snippet: Quantified proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membrane (Biorad). .. The membranes were blocked for 2 h with 1× Trisbuffered saline (TBS; Biorad) containing 5% Blotting-Grade Blocker (Biorad) and incubated overnight at 4 ◦C with primary antibodies against c-Myc (1:2000; 10828-1-AP; Proteintech Group, Inc., Rosemont, IL, USA), Cyclin D1 (1:1000; 2978S; Cell Signaling Technology), Ki-67 (1:500; ab16667; Abcam), Bax (1:5000; ab32503; Abcam), Caspase-9 (1:1000; 20750S; Cell Signaling Technology), NRF2 (1:1000; #12721; Cell Signaling Technology) and β-actin (1:1000; ab179467; Abcam). .. The following day, membranes were washed three times with 1× TBS containing 0.05% Tween-20 (Samchun) and incubated with secondary antibody (ab6728; Abcam), diluted 1:10,000, for 1 at room temperature.

    Article Title: The Comparison of Anticancer Efficiency of Methylene Blue- and Aluminum Phthalocyanine-Mediated Sonophotodynamic Therapy on Prostate Cancer Cells In Vitro.
    Article Snippet: Prostate cancer ranks highest in male diagnoses and second in cancer-related deaths.. Conventional treatments necessitate exploration of new modalities due to their side effects.. Sonophotodynamic therapy (SPDT) represents a potential anticancer approach that integrates both sonodynamic and photodynamic therapies to improve the efficacy of cancer treatments.

    Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death
    Article Snippet: The proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Amersham Bioscience). .. Subsequently. the membranes were subjected to overnight incubated at 4 ̊C with primary antibodies, including anti-human poly (ADP-ribose) polymerase (PARP, #9542s; Cell Signaling Technology, Inc.), caspases-3 (#9665s; Cell Signaling Technology, Inc.), caspase-9 (#9508s; Cell Signaling Technology, Inc.), Bax (NB100-56095; Novus Biologicals), Bcl-2 (NB100-56098; Novus Biologicals), p53 (sc-6243; Santa Cruz Biotechnology, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, sc-32233; Santa Cruz Biotechnology, Inc.). .. Following thorough washing with Tris-buffered saline, horseradish peroxidase-conjugated secondary antibodies, including anti-rabbit IgG or anti-mouse IgG (Invitrogen; Thermo Fisher Scientific, Inc.), were applied to the membranes at room temperature for 1 h. Specific bands were developed using the ImageQuant LAS 4000 chemiluminescence imaging equipment (GE Healthcare) and an Immunoblot detection kit from Bio-Rad.

    Article Title: Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HALO image analysis platform v3.6.4134.263 Indica Labs https://indicalab.com/halo/ Area Quantification v2.4.2 algorithm Indica Labs https://indicalab.com/halo/ CytoNuclear v2.0.9 algorithm (HALO) Indica Labs https://indicalab.com/halo/ CRISPOR Concordet et al.59 https://crispor.gi.ucsc.edu Image Lab Touch Software v. 2.4.0.03 BioRad https://www.bio-rad.com ImageJ64 software ImageJ https://imagej.net/ij/ Incucyte Software v. 2024B Sartorius https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software/incucyte-softwarev2024b Other Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer Thermo Fisher Scientific Cat# IQLAAEGAAPFADBMBHQ Easy-nLC 1200 LC System Thermo Fisher Scientific Cat# LC140 50 cm Emitters CoAnn Technologies Cat# ICT36007508-50 ABIRD - The Active Background Ion Reduction Device for nanospray LC/MS ESI Solutions Cat# ABIRD ll OPEN ACCESS Article e5 Molecular Cell 86, 1–13.e1–e11, April 16, 2026 membranes (Merck). .. Membranes were blocked with BSA 5% in TBST for 30 min and incubated with the following primary antibodies overnight (all 1:1000 dilution): ATG7 (Cell Signaling, Cat# 8558; RRID:AB_11179039), BiP (Cell Signaling, Cat# 3177; RRID:AB_2119845), β actin (Cell Signaling, Cat# 4970; RRID:AB_2223172), Caspase-2 (BD Transduction Laboratories, Cat# 611023; RRID:AB_397606), Caspase-8 (Cell Signaling, Cat# 4790; RRID:AB_10545723), Caspase-9 (Cell Signaling, Cat# 9502; RRID:AB_2228052), CHOP (Cell Signaling, Cat# 2895; RRID:AB_2089254), Cleaved caspase-3 (Asp175) (Cell Signaling, Cat# 9664; RRID:AB_2070042), Cleaved caspase 7 (Asp198) (Cell Signaling, Cat# 9491; RRID:AB_2068261), Cleaved caspase-8 (Cell Signaling, Cat# 9429; RRID:AB_2062634), Cleaved caspase-8 (Asp374) (for human samples) (Cell Signaling, Cat# 9496S; RRID:AB_561378), Cleaved caspase-8 (mouse specific) (Cell Signaling, Cat# 8592S; RRID:AB_10999739), Cleaved PARP (Asp214) (rodent specific) (Cell Signaling, Cat# 94885; RRID:AB_2800237), FADD (Abcam, Cat# 124812; RRID:AB_11155370), FADD (Merck, Cat# 05-486; RRID:AB_309984), FLAG (Sigma-Aldrich, Cat# F7425; RRID:AB_439687), FLAG (M2) mAb (Merck, Cat# F3165; RRID:AB_2209044), GAPDH (Abcam, Cat# ab9484; RRID:AB_307274), GFP (Santa Cruz Biotechnology, Cat# sc-9996; RRID:AB_627695), HA-tag (Merck, Cat# 11867423001; RRID:AB_390918), HSP90 (Santa Cruz, Cat# sc-1057; RRID:AB_675714), LC3B (Cell Signaling, Cat# 3868; RRID:AB_2137707), MLKL (Cell Signaling, Cat# 14993; RRID:AB_2687572), p62 in human cells (BD Transduction Laboratories, Cat# 610833; RRID:AB_398152), p62 in mouse cells (Sigma-Aldrich, Cat# P0067; RRID:AB_1841028), p-MLKL (Cell Signaling, Cat# 91689; RRID:AB_2732034), pRIPK1 S166 (rodent specific) (Cell Signaling, Cat# 31122; RRID:AB_2799000), p-RIPK1 (Ser166) (Cell Signaling, Cat# 65746; RRID:AB_2799693), RIPK1 (BD Transduction Laboratories, Cat# 610459; RRID:AB_398668), TRADD (Abcam, Cat# ab319029; RRID: AB_3741122), TRADD (Cell Signaling, Cat# 3694; RRID:AB_2209044). .. Vinculin (Sigma-Aldrich, Cat# V9131; RRID:AB_477629).

    Article Title: Caspase-3/7 deficiency results in enhanced intestinal inflammation and reduced tumorigenesis
    Article Snippet: Next, samples were mixed with Laemmli buffer, boiled at 95°C for 10 min, further loaded onto SDS–polyacrylamide gel electrophoresis gels, and transferred to a polyvinylidene difluoride membrane (Millipore). .. After blocking, proteins were probed by the following primary antibodies: caspase-1 (1:2000; VIB Core), caspase-3 (1:1000; 9662, CST), caspase-7 (1:1000; 9492S, CST), caspase-8 (1:1000; MAB3429, Abnova), clcaspase-8 (1:1000; 9429, CST), caspase-9 (1:1000; 9508, CST), caspase-11 (1:200; NB120-10454, NOVUS), pRIPK1 (Ser 166 ) (1:1000; 53286S, CST), RIPK1 (1:1000; 3493, CST), pRIPK3 (Thr 231 /Ser 232 ) (1:1000; 57220, CST), pMLKL (Ser 345 ) (1:1000; 37333, CST), MLKL (1:1000; MABC604, Millipore), GSDMD (1:1000; ab209845, Abcam), GSDME (1:500; 13075-1-AP, Proteintech), GPX4 (1:5000; ab125066, Abcam), vinculin (1:200; V9131, Sigma-Aldrich), β-tubulin HRP (1:10,000; ab21058, Abcam), and β-actin HRP (1:5000; 5125, CST); followed by HRP-conjugated secondary antibody (GE Healthcare) incubation. .. Detection was performed with the Western Lightning chemiluminescent reagent plus kit (NEL105001EA, PerkinElmer).

    Saline:

    Article Title: Antioxidant and Anti-Aging Effects of Porphyra-334 Produced from Saccharomyces cerevisiae in Human Skin Models.
    Article Snippet: Quantified proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membrane (Biorad). .. The membranes were blocked for 2 h with 1× Trisbuffered saline (TBS; Biorad) containing 5% Blotting-Grade Blocker (Biorad) and incubated overnight at 4 ◦C with primary antibodies against c-Myc (1:2000; 10828-1-AP; Proteintech Group, Inc., Rosemont, IL, USA), Cyclin D1 (1:1000; 2978S; Cell Signaling Technology), Ki-67 (1:500; ab16667; Abcam), Bax (1:5000; ab32503; Abcam), Caspase-9 (1:1000; 20750S; Cell Signaling Technology), NRF2 (1:1000; #12721; Cell Signaling Technology) and β-actin (1:1000; ab179467; Abcam). .. The following day, membranes were washed three times with 1× TBS containing 0.05% Tween-20 (Samchun) and incubated with secondary antibody (ab6728; Abcam), diluted 1:10,000, for 1 at room temperature.



    Similar Products

    94
    MedChemExpress z-lehd-fmk
    Z Lehd Fmk, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Z-LEHD-FMK/custom%40hy-p1010%4041887392
    Average 94 stars, based on 1 article reviews
    z-lehd-fmk - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    Boster Bio m00334
    M00334, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Anti-Caspase+3+Rabbit+Monoclonal+Antibody/pmc13085978-28-7-3
    Average 90 stars, based on 1 article reviews
    m00334 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Boster Bio pro caspase3 antibody
    Pro Caspase3 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Anti-Caspase+3+Rabbit+Monoclonal+Antibody/pmc13085978-28-0-3
    Average 90 stars, based on 1 article reviews
    pro caspase3 antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc anti cleaved caspase 9
    Anti Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Caspase-9+Antibody/pmc12666026-25-5-16
    Average 97 stars, based on 1 article reviews
    anti cleaved caspase 9 - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    86
    Wanleibio anti cleaved caspase 9 rabbit pab
    Anti Cleaved Caspase 9 Rabbit Pab, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/3+anti+caspase+cleaved/pm42106126-50-16-35
    Average 86 stars, based on 1 article reviews
    anti cleaved caspase 9 rabbit pab - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    94
    Bioss anti caspase 9
    Anti Caspase 9, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Caspase+9+Polyclonal+Antibody/pm42089455-121-38-34
    Average 94 stars, based on 1 article reviews
    anti caspase 9 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    86
    Affinity Biosciences cleaved caspase 9
    Cleaved Caspase 9, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/3+anti+caspase+cleaved/pmc12914111-39-8-70
    Average 86 stars, based on 1 article reviews
    cleaved caspase 9 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Abmart Inc cleaved caspase 9 asp353 antibody
    mBH3@NPs preferentially induced mtApoptosis and immunogenic cell death in cancer cells (A) Flow cytometry analysis of cells positive for Annexin V and propidium iodide (PI) in cancer and non-cancer cell lines after treatment with PBS (control), NPs, naked Puma mRNA, and mPuma@NPs for 12 h ( n = 3). (B) Western blot analysis of mitochondrial apoptois pathway in CT-26 cells treated with control, NPs, mPuma@NPs, and mBim@NPs. Bax, Bcl-2, Bcl-x L , Mcl-1, caspase-3, cleaved caspase-3 (C-Cas3), caspase9, and cleaved caspase-9 (C-Cas9) proteins were detected. β-Actin was used as the loading control ( n = 3). (C) Confocal laser scanning microscopy (CLSM) images of the 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine (JC-1) probe in CT-26 cells after treatment with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h. Increased JC-1 monomer signal (green) and decreased JC-1 aggregate signal (red) indicate a decrease in mitochondrial membrane potential ( n = 3). Scale bars, 200 μm. (D) Flow cytometry analysis of cellular oxygen species (ROS) levels using 2,7-dichlorofluorescein diacetate (DCFH-DA) staining in CT-26 cells after 12 h incubation with PBS (control), NPs, naked mRNA, mPuma@NPs, and mBim@NPs ( n = 3). (E) CLSM images of CRT expression in B16-F10 and CT-26 cells after 12 h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). Scale bars, 20 μm. (F) Extracellular ATP and HMGB1 expression levels were analyzed by ELISA in B16-F10 cells after 12-h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). (G) Flow cytometry analysis and quantification of immune stimulation in BMDCs co-cultured with B16-F10 cells pretreated with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h, followed by 48-h co-culture ( n = 3). One-way ANOVA with Tukey’s multiple comparisons test was used for all statistical analyses. Data are presented as the mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; NS, not significant. See also  .
    Cleaved Caspase 9 Asp353 Antibody, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/3+anti+caspase/pmc13130659-10-0-5
    Average 86 stars, based on 1 article reviews
    cleaved caspase 9 asp353 antibody - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc cleaved caspase 9 asp315 d8i9e rabbit mab
    CGF’s effect on cell cycle and apoptosis in CRC (A) Flow cytometry was used to analyze how CGF affects the cell cycle of HCT116 and HT29 cells at certain concentrations, with the percentage of cells in G1, S, and G2 phases shown in each panel. (B) Western blot analysis of the changes in cell cycle-related proteins CDK1, p-CDK1, and cyclin B1 in HCT116 and HT29 cells after CGF treatment. (C) RT-qPCR analysis of the relative expression levels of PUMA and NOXA genes in HCT116 and HT29 cells treated with different concentrations of CGF. (D) Western blot analysis of the changes in apoptosis-related proteins BCL2, PUMA, Noxa, C-caspase 9, and C-caspase 3 in HCT116 and HT29 cells after CGF treatment. (E) Flow cytometry was used to analyze apoptosis in HCT116 and HT29 cells treated with CGF. On the left is a representative plot showing apoptosis, utilizing Annexin V-FITC and PI double staining. Right: Analysis of early and late apoptosis in cells from each group using quantitative methods. (A–C and E) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Cleaved Caspase 9 Asp315 D8i9e Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Cleaved+Caspase-9+(Asp315)+Rabbit+mAb/pmc13018894-10-0-7
    Average 96 stars, based on 1 article reviews
    cleaved caspase 9 asp315 d8i9e rabbit mab - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beyotime caspase 9 activity kit
    CGF’s effect on cell cycle and apoptosis in CRC (A) Flow cytometry was used to analyze how CGF affects the cell cycle of HCT116 and HT29 cells at certain concentrations, with the percentage of cells in G1, S, and G2 phases shown in each panel. (B) Western blot analysis of the changes in cell cycle-related proteins CDK1, p-CDK1, and cyclin B1 in HCT116 and HT29 cells after CGF treatment. (C) RT-qPCR analysis of the relative expression levels of PUMA and NOXA genes in HCT116 and HT29 cells treated with different concentrations of CGF. (D) Western blot analysis of the changes in apoptosis-related proteins BCL2, PUMA, Noxa, C-caspase 9, and C-caspase 3 in HCT116 and HT29 cells after CGF treatment. (E) Flow cytometry was used to analyze apoptosis in HCT116 and HT29 cells treated with CGF. On the left is a representative plot showing apoptosis, utilizing Annexin V-FITC and PI double staining. Right: Analysis of early and late apoptosis in cells from each group using quantitative methods. (A–C and E) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Caspase 9 Activity Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+9/Caspase+9+Activity+Assay+Kit/pm41992349-113-25-29
    Average 96 stars, based on 1 article reviews
    caspase 9 activity kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    mBH3@NPs preferentially induced mtApoptosis and immunogenic cell death in cancer cells (A) Flow cytometry analysis of cells positive for Annexin V and propidium iodide (PI) in cancer and non-cancer cell lines after treatment with PBS (control), NPs, naked Puma mRNA, and mPuma@NPs for 12 h ( n = 3). (B) Western blot analysis of mitochondrial apoptois pathway in CT-26 cells treated with control, NPs, mPuma@NPs, and mBim@NPs. Bax, Bcl-2, Bcl-x L , Mcl-1, caspase-3, cleaved caspase-3 (C-Cas3), caspase9, and cleaved caspase-9 (C-Cas9) proteins were detected. β-Actin was used as the loading control ( n = 3). (C) Confocal laser scanning microscopy (CLSM) images of the 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine (JC-1) probe in CT-26 cells after treatment with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h. Increased JC-1 monomer signal (green) and decreased JC-1 aggregate signal (red) indicate a decrease in mitochondrial membrane potential ( n = 3). Scale bars, 200 μm. (D) Flow cytometry analysis of cellular oxygen species (ROS) levels using 2,7-dichlorofluorescein diacetate (DCFH-DA) staining in CT-26 cells after 12 h incubation with PBS (control), NPs, naked mRNA, mPuma@NPs, and mBim@NPs ( n = 3). (E) CLSM images of CRT expression in B16-F10 and CT-26 cells after 12 h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). Scale bars, 20 μm. (F) Extracellular ATP and HMGB1 expression levels were analyzed by ELISA in B16-F10 cells after 12-h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). (G) Flow cytometry analysis and quantification of immune stimulation in BMDCs co-cultured with B16-F10 cells pretreated with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h, followed by 48-h co-culture ( n = 3). One-way ANOVA with Tukey’s multiple comparisons test was used for all statistical analyses. Data are presented as the mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; NS, not significant. See also  .

    Journal: Cell Reports Medicine

    Article Title: mRNA lipid-nanoparticle-mediated mitochondrial apoptosis augments adoptive T cell immunotherapy

    doi: 10.1016/j.xcrm.2026.102706

    Figure Lengend Snippet: mBH3@NPs preferentially induced mtApoptosis and immunogenic cell death in cancer cells (A) Flow cytometry analysis of cells positive for Annexin V and propidium iodide (PI) in cancer and non-cancer cell lines after treatment with PBS (control), NPs, naked Puma mRNA, and mPuma@NPs for 12 h ( n = 3). (B) Western blot analysis of mitochondrial apoptois pathway in CT-26 cells treated with control, NPs, mPuma@NPs, and mBim@NPs. Bax, Bcl-2, Bcl-x L , Mcl-1, caspase-3, cleaved caspase-3 (C-Cas3), caspase9, and cleaved caspase-9 (C-Cas9) proteins were detected. β-Actin was used as the loading control ( n = 3). (C) Confocal laser scanning microscopy (CLSM) images of the 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine (JC-1) probe in CT-26 cells after treatment with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h. Increased JC-1 monomer signal (green) and decreased JC-1 aggregate signal (red) indicate a decrease in mitochondrial membrane potential ( n = 3). Scale bars, 200 μm. (D) Flow cytometry analysis of cellular oxygen species (ROS) levels using 2,7-dichlorofluorescein diacetate (DCFH-DA) staining in CT-26 cells after 12 h incubation with PBS (control), NPs, naked mRNA, mPuma@NPs, and mBim@NPs ( n = 3). (E) CLSM images of CRT expression in B16-F10 and CT-26 cells after 12 h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). Scale bars, 20 μm. (F) Extracellular ATP and HMGB1 expression levels were analyzed by ELISA in B16-F10 cells after 12-h incubation with PBS (control), NPs, mPuma@NPs, and mBim@NPs ( n = 3). (G) Flow cytometry analysis and quantification of immune stimulation in BMDCs co-cultured with B16-F10 cells pretreated with PBS (control), NPs, mPuma@NPs, and mBim@NPs for 12 h, followed by 48-h co-culture ( n = 3). One-way ANOVA with Tukey’s multiple comparisons test was used for all statistical analyses. Data are presented as the mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; NS, not significant. See also .

    Article Snippet: Cleaved-Caspase 9 (Asp353) Antibody , Abmart , Cat#TA5240S; RRID:AB_3740854.

    Techniques: Flow Cytometry, Control, Western Blot, Confocal Laser Scanning Microscopy, Membrane, Staining, Incubation, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Co-Culture Assay

    CGF’s effect on cell cycle and apoptosis in CRC (A) Flow cytometry was used to analyze how CGF affects the cell cycle of HCT116 and HT29 cells at certain concentrations, with the percentage of cells in G1, S, and G2 phases shown in each panel. (B) Western blot analysis of the changes in cell cycle-related proteins CDK1, p-CDK1, and cyclin B1 in HCT116 and HT29 cells after CGF treatment. (C) RT-qPCR analysis of the relative expression levels of PUMA and NOXA genes in HCT116 and HT29 cells treated with different concentrations of CGF. (D) Western blot analysis of the changes in apoptosis-related proteins BCL2, PUMA, Noxa, C-caspase 9, and C-caspase 3 in HCT116 and HT29 cells after CGF treatment. (E) Flow cytometry was used to analyze apoptosis in HCT116 and HT29 cells treated with CGF. On the left is a representative plot showing apoptosis, utilizing Annexin V-FITC and PI double staining. Right: Analysis of early and late apoptosis in cells from each group using quantitative methods. (A–C and E) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: CGF induces ROS-mediated metabolic reprogramming and mitochondrial dysfunction to suppress colorectal cancer progression

    doi: 10.1016/j.isci.2026.115273

    Figure Lengend Snippet: CGF’s effect on cell cycle and apoptosis in CRC (A) Flow cytometry was used to analyze how CGF affects the cell cycle of HCT116 and HT29 cells at certain concentrations, with the percentage of cells in G1, S, and G2 phases shown in each panel. (B) Western blot analysis of the changes in cell cycle-related proteins CDK1, p-CDK1, and cyclin B1 in HCT116 and HT29 cells after CGF treatment. (C) RT-qPCR analysis of the relative expression levels of PUMA and NOXA genes in HCT116 and HT29 cells treated with different concentrations of CGF. (D) Western blot analysis of the changes in apoptosis-related proteins BCL2, PUMA, Noxa, C-caspase 9, and C-caspase 3 in HCT116 and HT29 cells after CGF treatment. (E) Flow cytometry was used to analyze apoptosis in HCT116 and HT29 cells treated with CGF. On the left is a representative plot showing apoptosis, utilizing Annexin V-FITC and PI double staining. Right: Analysis of early and late apoptosis in cells from each group using quantitative methods. (A–C and E) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb , CST , Cat # 20750; RRID:AB_2798848.

    Techniques: Flow Cytometry, Western Blot, Quantitative RT-PCR, Expressing, Double Staining

    The effect of CGF-induced ROS on the MAPK/ERK 1/2/c-MYC signaling pathway (A) Western blotting was used to analyze the expression of MEK, P-MEK, ERK 1/2, P-ERK 1/2, and c-MYC proteins in HCT116 and HT29 cells after treatment with specified CGF concentrations. (B) Immunofluorescence staining of HCT116 and HT29 cells after CGF treatment, showing changes in p-ERK (green). PMA was used to promote nuclear entry of p-ERK (200 nM, 4 h), and DAPI was used to stain the nucleus (blue), with merged images shown (scale bars, 10 μM). (C) Western blot was used to analyze the levels of MEK, P-MEK, ERK 1/2, P-ERK 1/2, and c-MYC proteins in HCT116 and HT29 cells following treatment with CGF (40 μM, 24 h) and NAC (500 μM, 24 h). (D) Untargeted metabolomics analysis of glycolytic metabolites after CGF treatment in HCT116 cells. Metabolites marked in blue represent those inhibited by CGF. (E and F) ELISA analysis of lactate secretion in the cell supernatant of HCT116 (E) and HT29 (F) cells after CGF treatment. (G and H) Analysis of mRNA expression levels of genes related to glycolysis in HCT116 and HT29 cells exposed to certain concentrations of CGF using RT-qPCR. (I) Western blot was used to analyze the expression levels of apoptosis-associated proteins (BCL2, PUMA, NOXA, C-caspase 9, and C-caspase 3) and EMT markers (N-cadherin and vimentin) in HCT116 and HT29 cells after they were treated with CGF. (J) Western blot analysis of the reversal of EMT markers N-cadherin, vimentin, SLUG, and SNAIL proteins in HCT116 and HT29 cells overexpressing c-MYC. (E–J) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: CGF induces ROS-mediated metabolic reprogramming and mitochondrial dysfunction to suppress colorectal cancer progression

    doi: 10.1016/j.isci.2026.115273

    Figure Lengend Snippet: The effect of CGF-induced ROS on the MAPK/ERK 1/2/c-MYC signaling pathway (A) Western blotting was used to analyze the expression of MEK, P-MEK, ERK 1/2, P-ERK 1/2, and c-MYC proteins in HCT116 and HT29 cells after treatment with specified CGF concentrations. (B) Immunofluorescence staining of HCT116 and HT29 cells after CGF treatment, showing changes in p-ERK (green). PMA was used to promote nuclear entry of p-ERK (200 nM, 4 h), and DAPI was used to stain the nucleus (blue), with merged images shown (scale bars, 10 μM). (C) Western blot was used to analyze the levels of MEK, P-MEK, ERK 1/2, P-ERK 1/2, and c-MYC proteins in HCT116 and HT29 cells following treatment with CGF (40 μM, 24 h) and NAC (500 μM, 24 h). (D) Untargeted metabolomics analysis of glycolytic metabolites after CGF treatment in HCT116 cells. Metabolites marked in blue represent those inhibited by CGF. (E and F) ELISA analysis of lactate secretion in the cell supernatant of HCT116 (E) and HT29 (F) cells after CGF treatment. (G and H) Analysis of mRNA expression levels of genes related to glycolysis in HCT116 and HT29 cells exposed to certain concentrations of CGF using RT-qPCR. (I) Western blot was used to analyze the expression levels of apoptosis-associated proteins (BCL2, PUMA, NOXA, C-caspase 9, and C-caspase 3) and EMT markers (N-cadherin and vimentin) in HCT116 and HT29 cells after they were treated with CGF. (J) Western blot analysis of the reversal of EMT markers N-cadherin, vimentin, SLUG, and SNAIL proteins in HCT116 and HT29 cells overexpressing c-MYC. (E–J) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit mAb , CST , Cat # 20750; RRID:AB_2798848.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR